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Table 2 Gm40600 overexpression does not affect the mRNA expression of plasma cells-associated transcription factors and apoptosis-associated molecules

From: Gm40600 suppressed SP 2/0 isograft tumor by reducing Blimp1 and Xbp1 proteins

Gene Symbol

Total exon fragments

FPKM

Vector

Gm40600

Fold change

Vector

Gm40600

Fold change

Pax5

4.19

12

2.87

0.03

0.09

3

BCL6

171

127

0.74

2.55

2.07

0.81

Bach2

0

0

 

0

0

 

IRF4

14,695

12,090

0.82

163.91

147.68

0.9

Prdm1

2752

2584

0.94

31.65

33.71

1.07

Xbp1 (unspliced)

7731.6

8027.07

1.04

190.96

217.1

1.14

Xbp1 (spliced)

101.4

116.93

1.15

5.23

6.6

1.26

Trp53

4337

5386

1.24

127.9

173.83

1.36

Bcl2

34

18

0.53

0.27

0.14

0.52

Mcl1

21,732

26,477

1.22

406.54

576.35

1.42

  1. The stable Gm40600- and Vector-expressing SP 2/0 cells were cultured for 2 days in fresh medium. The transcripts were determined by RNA-sequencing. Total exon fragments and FPKM (FPKM = total exon fragments / mapped reads (millions) / exon length (KB), Fragments per Kilo bases per Million reads) values of selected genes encoding Pax5, Bach2, Bcl6, Irf4, Prdm1, unspliced and spliced Xbp1, Trp53, Bcl2, and Mcl1, and the fold change of gene expression in Gm40600-expressing SP 2/0 cells to that in vector-expressing SP 2/0 cells are shown