Fig. 3

Gm40600 overexpression suppressed the PC-related proteins Blimp1 and Xbp-1 in SP 2/0 cells. a Gm40600 localized to the cytoplasm. The stable Gm40600-EGFP (Gm40600)-, or EGFP alone (Vector)-expressing SP 2/0 cells were cultured for 2 days. EGFP were imaged in cells on a GE IN Cell Analyzer 2000. Representative images show cytoplasm localization of Gm40600. b Gm40600 overexpression suppressed the PC-related proteins Blimp1 and Xbp-1 in SP 2/0 cells. Gm40600- or vector-expressing SP 2/0 cells described in Figs. 1 and 2 were cultured for 2 days and followed by immunoblot analysis using monoclonal anti-mouse Bcl6, IRF4, Blimp1, Xbp1, Trp53, Bcl2, and β-tubulin antibodies. Results represent three independent experiments. c Blimp1 promoted the activation of the Bcl2 promoter. The Blimp1-expressing lentiviral vector LV81 (Blimp1) or empty vector Lv81 (Vector) and the luciferase reporter vector pEZX-PG04.1/Bcl2 promoter (− 1323 ~ + 160 bp) were co-transduced into 293 T cells. Dual luciferase reporter gene expression was analyzed, and the results are shown as the ratio of firefly to renilla luciferase activity. The data represent at least four independent experiments. Error bars, SEM. Two tailed Student’s t-test, *P < 0.05