External validation of molecular subtype classifications of colorectal cancer based on microsatellite instability, CIMP, BRAF and KRAS

Table 1 Characteristics of studies for validation

	Studies with proposed classifications to be validated			Validation set
	Samadder et al. [20] 2013	Phipps et al. [21] 2015	Sinicrope et al. [16] 2015	Validation set
Study	Iowa Women’s Health Study	Seattle Colon Cancer Family Registry	Phase III randomized trial NCCTG N0147	DACHS study
Study design	Cohort	Population-based cohort	Cohort from clinical trial	Population-based cohort
N	370	1189	2720	1915
Country	US	US	US	Germany
Population	Women aged 55–69 resident in Iowa	Persons aged 20–74 resident in Washington state and women 50–74 in postmenopausal study	Patients aged 19–86 with resected stage III colon carcinomas in Alabama region	Patients aged 30–96 resident in Rhine-Neckar region
Cancer	Colorectal	Colorectal	Colon	Colorectal
Stage	I - IV	I - IV	III	I - IV
Recruitment time	1986–2002	1998–2007	2004–2012	2003–2010
Treatment	Any	Any	FOLFOX or FOLFOX+cetuximab	Any
Outcomes	CSS, OS	CSS, OS	DFS	CSS, RFS, OS
Median follow-up	NR	NR	4.9 years	5.3 years

DACHS Darmkrebs, Chancen der Verhütung durch Screening, US United States, CSS cancer-specific survival, DFS disease-free survival, OS overall survival, NR not reported

ISSN: 1471-2407