Fig. 4

Effect of fish oil diet on the growth of miR-23b and miR-27b knockout cell orthotopic tumor xenografts. Tumor xenograft growth curve of empty vector (a), miR-23b (b) and miR-27b (c) knockout xenograft mice fed a fish-oil based diet versus a standard diet; n = 6 mice per group, ** p < 0.01, ****p < 0.0001, Two-way ANOVA. Survival analysis of empty vector (d), miR-23b (e) and miR-27b (f) knockout xenograft mice fed a fish-oil based diet versus a standard diet. Survival curves were compared using the Log-rank (Mantel-Cox) test in GraphPad Prism g. Representative images and h. quantitation of immunohistochemistry staining CD31 positive microvessels in xenograft tumor specimens, black bar = 100 μm; n = 3 per group, *p < 0.05, ** p < 0.01, Student’s t-test. Blue arrows indicate representative regions of CD31 positive microvessels