Fig. 2

miR-23b and miR-27b knockout cell growth and migration characteristics. Cellular growth curve of miR-23b (a) and miR-27b (b) knockout clones. Cells were plated in quadruplicate and proliferation was measured by MTS assay, *p < 0.05, ** p < 0.01, **** p < 0.0001, Student’s t-test. Cell migration of miR-23b (c) and miR-27b (d) knockout cells was measured compared to empty vector control cells by the wound healing assay. A wound was made in a monolayer of cells and migration into the wound was measured every 24 h for a total of 72 h. Representative images (20X magnification) of the wound were captured (upper) and the rate of wound closure was calculated (lower); n = 6 per time point, ** p < 0.01, **** p < 0.0001, Two-way ANOVA. Clonogenic assay of miR-23b (e) and miR-27b (f) knockout cells compared to empty vector control cells. Soft-agar assay of miR-23b (g) and miR-27b (h) knockout cells compared to empty vector control cells. Representative images of colony growth and colony counts of the average of 2 experiments performed in triplicate; *p < 0.05, ** p < 0.01, ***p < 0.001, **** p < 0.0001, One-way ANOVA, Dunnett’s post-test