Table 1 Quantitative real-time PCR primer sequences
Target | Forward 5’ → 3’ | Reverse 5’ → 3’ |
|---|---|---|
β-actin | CATGTACGTTGCTATCCAGGC | CTCCTTAATGTCACGCACGAT |
PLA2G4A | GAAAAGTGGGCTAAAATGAACAAG | GGGCAATCTTTCTCCATATCAG |
COX1 | CGTGTGTGTGACCTGCTGAA | GTACTCCTCGATGACAATCTTGATG |
COX2 | CAGGCTTCCATTGACCAGAG | TTTCTCCTGTAAGTTCTTCAAATGAT |
PTGES | GTGGCTATACCTGGGGACTT | AATCCAAGGGGCTAAGAAACAT |
EP1 | ATCTGCTGGAGCCCAATGC | GATCTGGTTCCAGGAGGCAA |
EP2 | CAGACCCTGGTGGCACTG | CGAAGAGCATGAGCATCGTG |
EP3 | TCAATCAGACATCAGTTGAGCAC | TTTCTTAACAGCAGGTAAACCCAA |
EP4 | AGTTTGGAGCGAGAAGTCAGTA | GCGGCAGAAGAGGCATTTG |
15-PGDH | AGTAGTGAACATCAATGAACATCTGA | ACTGGGCAAACCGACATCAT |
PGT | GCCACAGCAGATGAAGCAAG | CCACCAGGACGAAGAGTGAG |
ERK1 | AGAGATCATGCTGAACTCCAAG | GTGCTTGCCAGGGAAGATG |
ERK2 | TGGATGTGGTGTTATGGAAAGA | AAGCAGAGACGCAGAATGAC |
AKT1 | GGCACCCCTTCCTCACAG | GGCGTACTCCATGACAAAGC |
AKT2 | CTGCGGAAGGAAGTCATCATT | GGTCGTGGGTCTGGAAGG |
β-catenin | CCATTACAACTCTCCACAACCT | GAGCAAGGCAACCATTTTCTG |
GSK3B | AAGTAATCCACCTCTGGCTACC | AGAAGCAGCATTATTGGTCTGTC |
PPP2R1B | GGTTCACCTCTCGCACATCT | TCTGAGCACAAGGAACGGAAT |
PTPRM | ACTCGTTGCCACAGTTATAATCTC | GTGTATGGTGACAGGTTAGTGATC |