|
shRNA1
|
GCTGATGGTTTATGCCACAAGTTGA
|
|
Top strand
|
GATCCGCTGATGGTTTATGCCACAAGTTGATTCAAGAGATCAACTTGTGGCATAAACCATCAGCTTTTTTC
|
|
Bottom strand
|
AATTGAAAAAAGCTGATGGTTTATGCCACAAGTTGATCTCTTGAATCAACTTGTGGCATAAACCATCAGCG
|
|
shRNA2
|
GTCACCGAGATCTTCGGGCTGCTAAT
|
|
Top strand
|
GATCCGTCACCGAGATCTTCGGGCTGCTAATTTCAAGAGAATTAGCAGCCCG AAGATCTCGGTGATTTTTTC
|
|
Bottom strand
|
AATTGAAAAAATCACCGAGATCTTCGGGCTGCTAATTCTCTTGAAATTAGCA GCCCGAAGATCTCGGTGACG
|
|
NC-shRNA
|
TTCTCCGAACGTGTCACGTAA
|
|
Top strand
|
GATCCGTTCTCCGAACGTGTCACGTAATTCAAGAGATTACGTGACACGTTCGGAGAATT TTTTC
|
|
Bottom strand
|
AATTGAAAAAATTCTCCGAACGTGTCACGTAATCTCTTGAATTACGTGACACGTTCGGAG AACG
|
|
cDNA
|
|
YES1-F
|
CGAATTCCCCCGGGCGGGATCCGCCACCATGGGCTGCATTAAAAGTA
|
|
YES1-R
|
CGGCCGCTACGCGTCGGGGTACCTAAATTTTCTCCTGGCTGGTACT
|
