Fig. 4
a Targeted silencing of YAP inhibits the expression of mRNA and protein levels. a qRT-PCR was used to analyse the mRNA expression of YAP, GSK-3β, DDK1, E-cadherin, β-catenin and vimentin in Hep-2 cell lines after transfection or not, and GAPDH was used as the internal control. Experiments were repeated ten times, and data are shown as the mean ± SD, **P < 0.01. b Western blot assay was employed to investigate the expression of YAP, β-catenin, E-cadherin, vimentin, DDK1 and GSK-3β. WB results indicated that the expression of vimentin and β-catenin were downregulated with YAP knockdown and were upregulated when cells were administered YAP-cDNA. Representative images are shown. Statistical analysis of the relative optical density of each band is shown. GAPDH was used as an internal control, **P < 0.01. c Model of the role of YAP in laryngeal cancer