Fig. 2

YAP knockdowned suppresses cell proliferation, invasion and migration. Silenced YAP inhibited cell proliferation in Hep-2 cells by CCK-8 (top panel). a The OD value at 450 nm for 5 days. Hep-2 with no treatment (Control), Non-nonspecific shRNA treatment (YAP-NC), YAP knockdown (YAP-shRNA) groups, and cDNA overexpression after YAP knockdown (YAP-shRNA+YAP-cDNA), *P < 0.05. b Colony formation assays (second panel) were performed to evaluate the proliferative capability of control, YAP-NC, YAP-shRNA and YAP-shRNA+YAP-cDNA cell groups. A representative image is shown, and a statistical comparison of the indicated groups was performed across four independent experiments, **P < 0.01. c Wound healing assays (third panel) were performed to explore the migration capability, and solid lines represented the wound edges. Images were captured by using light microscopy (40X). The migration index was calculated as described in the Materials and Methods (control, YAP-NC, YAP-shRNA and YAP-shRNA+YAP-cDNA). Statistical analysis is shown, **P < 0.01