Fig. 1

YAP knockdowned significantly decreased YAP expression at mRNA (a) and protein (b) levels in Hep-2 cells. c Immunofluorescence (bottom panel) was used to detect the expression of YAP (red). DAPI (blue) was used to stain the nuclei. The fluorescence intensity of YAP was stronger in YAP-NC groups, and was weaker in cells transfected with shRNA. One representative experiment out of the three performed is shown (80X). Experiments were repeated three times, and data are shown as the mean ± SD, **P < 0.01 (a), **P < 0.01 (b)