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Fig. 3 | BMC Cancer

Fig. 3

From: Elevated levels of circulating ITIH4 are associated with hepatocellular carcinoma with nonalcoholic fatty liver disease: from pig model to human study

Fig. 3

Protein identifications from the BN/SDS 2D-PAGE gels by MALDI-TOF MS/MS and validation for serum ITIH4, ceruloplasmin, and haptoglobin. a ITIH4, ceruloplasmin (Cp), and haptoglobin (Hpt) were identified from protein spots on a vertical line in the NAFLD group from the BN/SDS 2D-PAGE gels by MALDI-TOF MS/MS. b Chronological changes of serum expression of ITIH4, ceruloplasmin, and haptoglobin in the NAFLD group and control animal by western blotting. Cropped blots are showed in the figure. Full-length blots are presented in Additional file 4: Figure S3. c Chronological changes of serum ITIH4 levels by enzyme-linked immunosorbent assay. ITIH4 levels in the NAFLD group are expressed as the mean values. The mean values for 0, 12, 24, 36, and 60 weeks in the NAFLD group and the control were 0.48, 0.45, 0.73, 0.77, and 1.70 ng/ml, and 0.60, 0.44, 0.49, 0.34, and 0.01 ng/ml, respectively. Inter-α-trypsin inhibitor heavy chain 4: ITIH4, ceruloplasmin: Cp, haptoglobin: Hpt

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