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Fig. 2 | BMC Cancer

Fig. 2

From: Inhibition of PI3K/Akt/mTOR signaling pathway alleviates ovarian cancer chemoresistance through reversing epithelial-mesenchymal transition and decreasing cancer stem cell marker expression

Fig. 2

EOC-cis cells showed higher expressions of EMT and CSC markers. a mRNA expressions of EMT markers were detected by qRT-PCR. GAPDH was used as a control. b Typical images of immnuflourescence for the expressions of EMT markers in EOC-cis and parental cells were obtained at 200x magnification (Green). Red indicates nuclei stain. c Protein expressions of EMT markers were detected by western blot. β-actin was used as a loading control. d mRNA expressions of CSC markers in EOC-cis cell lines were detected by qRT-PCR. GAPDH was used as a control. e Typical images of immnuflourescence for the expressions of CSC markers in EOC-cis and parental cells were obtained at 200x magnification (Green). Red indicates nuclei stain. f Protein expressions of CSC markers were detected by western blot. β-actin was used as a loading control. All data were expressed as mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001 versus parental cells (n = 3)

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BMC Cancer

ISSN: 1471-2407

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