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Fig. 3 | BMC Cancer

Fig. 3

From: Long non-coding RNA HOXB-AS3 promotes myeloid cell proliferation and its higher expression is an adverse prognostic marker in patients with acute myeloid leukemia and myelodysplastic syndrome

Fig. 3

HOXB-AS3 regulates genes involving in cell cycle progression and DNA replication instead of HOX clusters. a The expressions of HOX genes were not influenced by HOXB-AS3 expression. RNA was purified from OCI/AML3 infected by lentivirus carrying pLKO-shLacZ (control#1), pLKO-vector (control#2), pLKO-shHOXB-AS3#1 or pLKO-shHOXB-AS3#2. b Differentially expressed genes between the control cells and HOXB-AS3 knockdown cells were revealed by microarray analysis. c GSEA analysis of the differentially expressed genes in HOXB-AS3 knockdown cells compared to the control cells. d Quantitative PCR analysis of the expressions of indicated genes in OCI/AML3 cells infected with lentivirus carrying pLKO-vector (control), shHOXB-AS3#1, or shHOXB-AS3#2. Data were derived from the triple repeats of experiments. P values were calculated by Kruskal-Wallis test. e Quantitative real time PCR analysis of RNA18S5 RNA (18 s rRNA), HOXB-AS3, MALAT1, NEAT1 and GAPDH in the nuclear and cytoplasmic fractions of OCI/AML3 cells. (* meant that P value was less than 0.05)

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