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Fig. 4 | BMC Cancer

Fig. 4

From: PRAF2 overexpression predicts poor prognosis and promotes tumorigenesis in esophageal squamous cell carcinoma

Fig. 4

Impact of PRAF2 expression on ESCC cell invasion and apoptosis. a The effect of PRAF2 on cell invasion was determined by transwell assay, using cells with PRAF2 silencing. b The number of cells that migrated through the membranes was calculated and depicted in the bar chart. c Flow cytometry assay was performed to analyze apoptosis of TE1 cells with PRAF2 knockdown. d The bar chart represented the percentage of apoptosis. e The protein levels of apoptosis regulators were examined by western blot. f The relative densities of proteins were quantified and normalized to GAPDH. Values represented the mean ± SD data in triplicate. *P < 0.05 and **P < 0.01

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