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Table 2 Estimated composition of 46 N-linked glycans identified from human serum glycoproteins

From: Exploring serum and immunoglobulin G N-glycome as diagnostic biomarkers for early detection of breast cancer in Ethiopian women

Pea#

m/z

Glycan composition

Type

In IgG

1

1362.48109

(Hex)2 + (Man)3 (GlcNAc)2

High-Mannose

 

2

1444.53419

(HexNAc)2 + (Man)3 (GlcNAc)2

Complex

+

3

1524.53392

(Hex)3 + (Man)3 (GlcNAc)2

High-Mannose

+

4

1565.56047

(Hex)2 (HexNAc)1 + (Man)3 (GlcNAc)2

Hybrid

+

5

1590.59210

(HexNAc)2 (Deoxyhexose)1 + (Man)3 (GlcNAc)2

Complex

+

6

1606.58702

(Hex)1 (HexNAc)2 + (Man)3 (GlcNAc)2

Complex

+

7

1686.58675

(Hex)4 + (Man)3 (GlcNAc)2

High-Mannose

 

8

1708.61871

(Hex)1 (HexNAc)1 (NeuAc)1 + (Man)3 (GlcNAc)2

Complex

 

9

1752.64493

(Hex)1 (HexNAc)2 (Deoxyhexose)1 + (Man)3 (GlcNAc)2

Complex

+

10

1768.63985

(Hex)2 (HexNAc)2 + (Man)3 (GlcNAc)2

Complex

+

11

1793.67148

(HexNAc)3 (Deoxyhexose)1 + (Man)3 (GlcNAc)2

Complex

+

12

1848.63958

(Hex)5 + (Man)3 (GlcNAc)2

High-Mannose

 

13

1854.67662

(Hex)1 (HexNAc)1 (Deoxyhexose)1 (NeuAc)1 + (Man)3 (GlcNAc)2

Complex

 

14

1870.67154

(Hex)2 (HexNAc)1 (NeuAc)1 + (Man)3 (GlcNAc)2

Hybrid

+

15

1911.69809

(Hex)1 (HexNAc)2 (NeuAc)1 + (Man)3 (GlcNAc)2

Complex

 

16

1914.69776

(Hex)2 (HexNAc)2 (Deoxyhexose)1 + (Man)3 (GlcNAc)2

Complex

+

17

1955.72431

(Hex)1 (HexNAc)3 (Deoxyhexose)1 + (Man)3 (GlcNAc)2

Complex

+

18

2010.69241

(Hex)6 + (Man)3 (GlcNAc)2

High-Mannose

 

19

2032.72437

(Hex)3 (HexNAc)1 (NeuAc)1 + (Man)3 (GlcNAc)2

Hybrid

 

20

2057.75600

(Hex)1 (HexNAc)2 (Deoxyhexose)1 (NeuAc)1 + (Man)3 (GlcNAc)2

Complex

+

21

2073.75092

(Hex)2 (HexNAc)2 (NeuAc)1 + (Man)3 (GlcNAc)2

Complex

 

22

2117.77714

(Hex)2 (HexNAc)3 (Deoxyhexose)1 + (Man)3 (GlcNAc)2

Complex

+

23

2175.78261

(Hex)2 (HexNAc)2 (NeuAc)2 + (Man)3 (GlcNAc)1

I.S

 

24

2219.80883

(Hex)2 (HexNAc)2 (Deoxyhexose)1 (NeuAc)1 + (Man)3 (GlcNAc)2

Complex

+

25

2260.83538

(Hex)1 (HexNAc)3 (Deoxyhexose)1 (NeuAc)1 + (Man)3 (GlcNAc)2

Complex

 

26

2263.83505

(Hex)2 (HexNAc)3 (Deoxyhexose)2 + (Man)3 (GlcNAc)2

Complex

 

27

2276.83030

(Hex)2 (HexNAc)3 (NeuAc)1 + (Man)3 (GlcNAc)2

Complex

 

28

2336.85144

(Hex)3 (HexNAc)4 + (Man)3 (GlcNAc)2

Complex

 

29

2378.86199

(Hex)2 (HexNAc)2 (NeuAc)2 + (Man)3 (GlcNAc)2

Complex

 

30

2422.88821

(Hex)2 (HexNAc)3 (Deoxyhexose)1 (NeuAc)1 + (Man)3 (GlcNAc)2

Complex

+

31

2438.88313

(Hex)3 (HexNAc)3 (NeuAc)1 + (Man)3 (GlcNAc)2

Complex

 

32

2483.89335

(Hex)3 (HexNAc)1 (Deoxyhexose)1 (NeuAc)2 + (Man)3 (GlcNAc)2

Hybrid

 

33

2520.93623

(Hex)1 (HexNAc)5 (NeuAc)1 + (Man)3 (GlcNAc)2

Complex

 

34

2524.91990

(Hex)2 (HexNAc)2 (Deoxyhexose)1 (NeuAc)2 + (Man)3 (GlcNAc)2

Complex

 

35

2581.94137

(Hex)2 (HexNAc)3 (NeuAc)2 + (Man)3 (GlcNAc)2

Complex

 

36

2584.94104

(Hex)3 (HexNAc)3 (Deoxyhexose)1 (NeuAc)1 + (Man)3 (GlcNAc)2

Complex

 

37

2727.99928

(Hex)2 (HexNAc)3 (Deoxyhexose)1 (NeuAc)2 + (Man)3 (GlcNAc)2

Complex

 

38

2743.99420

(Hex)3 (HexNAc)3 (NeuAc)2 + (Man)3 (GlcNAc)2

Complex

 

39

2890.05211

(Hex)3 (HexNAc)3 (Deoxyhexose)1 (NeuAc)2 + (Man)3 (GlcNAc)2

Complex

 

40

3007.09472

(Hex)4 (HexNAc)5 (NeuAc)1 + (Man)3 (GlcNAc)2

Complex

 

41

3049.10527

(Hex)3 (HexNAc)3 (NeuAc)3 + (Man)3 (GlcNAc)2

Complex

 

42

3109.12641

(Hex)4 (HexNAc)4 (NeuAc)2 + (Man)3 (GlcNAc)2

Complex

 

43

3195.16318

(Hex)3 (HexNAc)3 (Deoxyhexose)1 (NeuAc)3 + (Man)3 (GlcNAc)2

Complex

 

44

3414.23748

(Hex)4 (HexNAc)4 (NeuAc)3 + (Man)3 (GlcNAc)2

Complex

 

45

3560.29539

(Hex)4 (HexNAc)4 (Deoxyhexose)1 (NeuAc)3 + (Man)3 (GlcNAc)2

Complex

 

46

3719.34855

(Hex)4 (HexNAc)4 (NeuAc)4 + (Man)3 (GlcNAc)2

Complex

 

47

3865.40646

(Hex)4 (HexNAc)4 (Deoxyhexose)1 (NeuAc)4 + (Man)3 (GlcNAc)2

Complex

 
  1. Peak number 23 is an internal standard (I.S: disialyloctasaccharide). The “+” sign in the last column indicates those glycans which were also detected in IgG fraction of the samples