Table 1 TLR antibodies used and TLR staining patterns in NPC and normal controls
From: Expression of toll-like receptors in non-endemic nasopharyngeal carcinoma
Antigen | Primary antibody | Dilution | Staining pattern in NPC | Staining pattern in normal nasopharyngeal epithelial cells |
|---|---|---|---|---|
TLR1 | sc-30,000, polyclonal, rabbit, Santa Cruz Biotechnology Inc., CA, USA | 1:100 | Cytoplasmic | Cytoplasmic |
TLR2 | sc-10,739, polyclonal, rabbit, Santa Cruz Biotechnology Inc., CA, USA | 1:200 | Nuclear and cytoplasmic | Nuclear and cytoplasmic |
TLR4 | sc-10,741, polyclonal, rabbit, Santa Cruz Biotechnology Inc., CA, USA | 1:300 | Cytoplasmic | Nuclear and cytoplasmic |
TLR5 | NBP2–24787, monoclonal, mouse, Novus Biologicals, CO, USA | 1:100 | Nuclear membranous and cytoplasmic | Nuclear membranous and cytoplasmic |
TLR7 | IMG-581A, polyclonal, rabbit, Imgenex/Novus Biologicals, CO, USA | 1:300 | Nuclear membranous and nuclear | Nuclear and cytoplasmic |
TLR9 | sc-25,468, polyclonal, rabbit, Santa Cruz Biotechnology Inc., CA, USA | 1:100 | Cytoplasmic | Plasma membranous and cytoplasmic |