Fig. 1

The expression and hypermethylation of hsa-miR-124-3p in ESCC tissues and cells. The mRNA expression of hsa-miR-124-3p in normal tissues and ESCC tissues (a) and its expression in HEEC, KYSE-150 and Eca109 cells (b) were determined by qRT-PCR analysis. The methylation status of hsa-miR-124-3p in normal tissues and ESCC tissues (c) and its status in HEEC, KYSE-150 and Eca109 cells (d) were determined by bisulfite Sanger sequencing and methylation specific PCR respectively. hsa-miR-124-3p expression in tumor tissues was shown in the patents with different TNM stages (e) and different differentiation (f). Experiments were performed in triplicate and each value represents mean ± SD. ^***P < 0.001