Fig. 5
From: Prognostic value of TOP2A in bladder urothelial carcinoma and potential molecular mechanisms
Apoptosis analysis after TOP2A knockdown. a Apoptosis were significantly increased after TOP2A knockdown in J82 and cells after induction of apoptosis by gemcitabine treatment for 24 h. b Western blotting assay revealed increasing expression of cleaved caspase 3 after TOP2A knockdown. c-d Dose response curves of bladder cancer cell lines treated with doxorubicin at different concentrations as determined in 72-h cell viability assays. NE = necrosis; NC = negative control; IC50 = half maximal inhibitory concentration *, p < 0.05. At least three independent replicates were performed for each experiment