Fig. 1

Increased TOP2A expression in bladder urothelial carcinoma (BLCA) revealed by RNA-sequencing (a) and RT-qPCR (c-e). a Hierarchical clustering heat map of differentially expressed genes between tumor tissue and matched normal epithelial tissue. b Expression levels of TOP2A in each sample for RNA-sequencing. c TOP2A gene expression was significantly up regulated in tumor tissue compared with matched normal epithelial tissue. d TOP2A gene expression in different stages of BLCA. e TOP2A gene expression in different grades of BLCA. f TOP2A gene expression was significantly higher in MIBC compared with NMIBC in NCBI-GEO GSE31684 dataset. MIBC = muscle-invasive bladder cancer; NMIBC = non-muscle invasive bladder cancer; RPKM = reads per gene per kilobase exon per million mapped reads. **, p < 0.01