Fig. 4

EMILIN1 and TSPAN9 have synergistic anti-tumor effects. a A heatplot map of gene expression levels of the top 15 upregulated genes in GC tissues. b TSPAN9 and EMILIN1 plasmids were transfected into SGC7901 cells for 24 h, and immunofluorescence was employed to establish the location of TSPAN9 and EMILIN1 in SGC7901 cells. c SGC7901 cells were transfected with TSPAN9 and EMILIN1 plasmids, and for co-immunoprecipitation of TSPAN9 complexes was then conducted in whole cell protein lysates. Immunoprecipitated products were separated via SDS-PAGE and stained using silver staining reagent. The extracted peptide was sequenced by mass spectrometry. TSPAN9 plasmids or TSPAN9 and EMILIN1 plasmids were transfected into SGC7901 cells, and then wound-healing (d) and migration assays (e) were conducted to assess how TSPAN9 and TSPAN9 + EMILIN1 affect GC cell migration. *p < 0.05, **p < 0.01, Student’s t test