Fig. 3

The FAK-RAS-ERK1/2 pathway is activated via the low expression of TSPAN9. a In SGC7901 cells transfected with a TSPAN9 inhibitor (si-RNA) or a negative control (control) for 48 h, FAK, HRAS, and ERK1/2 levels were assessed by western blotting, with β-actin as a loading control. b and c si-TSPAN9 or si-TSPAN9 and RAS inhibitor was transfected into SGC7901 cells. We them conducted wound-healing (d) and migration assays (e) to assess how TSPAN9 siRNA and RAS inhibition affect the migration of GC cells. *p < 0.05, **p < 0.01, Student’s t test