Fig. 7
From: Blocking podoplanin suppresses growth and pulmonary metastasis of human malignant melanoma
SZ168 detects hPDPN expression in the lung micro-metastasis foci by immunohistochemistry. Lung sections were stained with H&E (a, d). Scale bar, 200 μm. Arrows indicate cancer nest. Sections were incubated with (b, e) SZ168 or (c, f) 18H5 antibody as well followed by the Envision+ kit reagents. Color was developed using DAB and then counterstained with hematoxylin. Scale bar, 20 μm. Arrows indicate cancer nest