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Fig. 4 | BMC Cancer

Fig. 4

From: Blocking podoplanin suppresses growth and pulmonary metastasis of human malignant melanoma

Fig. 4

SZ168 detects hPDPN expression in the lung micro-metastasis foci and subcutaneous tumors in IHC analysis. The subcutaneous tumors (a) and lungs (d, g) were stained with H&E. Scale bar, 20 μm. Arrows indicate cancer nest. The subcutaneous tumors and lungs were incubated with SZ168 (b, e, h) or 18H5 (c, f, i) and then by the Envision+ kit reagents. Color was developed using DAB and subsequently counterstained with hematoxylin. Scale bar, 200 or 20 μm. Arrows indicate cancer nest

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