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Fig. 8 | BMC Cancer

Fig. 8

From: A new mouse model to study the role of ectopic Nanos3 expression in cancer

Fig. 8

Differentiated metastases in lymph nodes of athymic mice injected with Nanos3-expressing tumor-derived cell cultures. Sections of H&E stained lymph nodes of mice injected with LuTDco or LuTDNa3 cell cultures showed the presence of, respectively, infrequent atypical cells lacking differentiation features (top panels, arrows), and prominent differentiated metastatic lesions (bottom panels). Lymph node sections were stained for E-cadherin and pan-cytokeratin, providing proof for the epithelial origin and the obvious differentiation of the lymph node metastases. Staining of lymph node sections with a GFP-specific antibody confirmed that the nodules in the lymph nodes of the LuTDNa3-injected mice were derived from the transgene-positive primary tumors, as expected. The negative controls represent sections stained with secondary antibodies only. Bars: 50 μm

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