Fig. 7
From: A new mouse model to study the role of ectopic Nanos3 expression in cancer
The effects of Nanos3 on the malignant behavior of lung tumor-derived cells. a. Soft agar analysis. Cell cultures derived from the lungs of a LSL-KRasG12D;p53fl/fl;CCSP-rtTA+/−;TetO-Cre+/− mouse (control NSCLC) and a Nanos3LSL/−;LSL-KRasG12D;p53fl/fl;CCSP-rtTA+/−;TetO-Cre+/− mouse (Nanos3 NSCLC) were grown in a soft agar solution at a density of 104 cells/ml. Parental and N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) transformed HOS (human osteosarcoma) cell cultures were used as a negative and a positive control, respectively. Micrographs were quantified with Volocity. Only colonies bigger than 100 μm2 were taken into account. Error bars, SEM; ns: not significant; **: P ≤ 0.01. b. Allograft experiment. Five athymic mice were each subcutaneously injected with 2.5 × 106 cells of a culture derived from the lungs of either a control NSCLC or a Nanos3-overexpressing NSCLC mouse. Tumor volumes were measured twice a week. Each graph depicts the average tumor growth curve of mice injected with the same cell culture