Fig. 2
From: A new mouse model to study the role of ectopic Nanos3 expression in cancer
Analysis of the livers of Alb-Cre;Nanos3 mice (a, b) and skin from K5-Cre;Nanos3 mice (c, d) with expression of heterozygous or homozygous Nanos3LSL alleles. Western blot (a) and RT-qPCR analysis (b) of liver lysates from control mice and mice with liver-specific expression of heterozygous or homozygous ectopic Nanos3LSL alleles. c. RT-qPCR analysis was done to check for eGFP and NANOS3 RNA expression in RNA lysates of skin from a Nanos3LSL/−;K5-Cre+/− mouse and a Nanos3LSL/−;K5-Cre−/− mouse. CNRQ, calibrated normalized relative quantity; error bars, SEM; n = 3. b and c. Gene expression was normalized to reference genes (Tbp and Hmbs) using qbase+ (Biogazelle) [35]. d. eGFP expression in skin sections from a Nanos3LSL/−;K5-Cre−/− mouse and a Nanos3LSL/LSL;K5-Cre+/− mouse was analyzed by immunohistochemical staining. Bars; 100 μm