Fig. 5
Measurement of FN content in PSC-CM and its effect on gemcitabine uptake by PCCs. a Amount of soluble FN in conditioned medium from ten different PSC cultures was measured by ELISA. b Both PCCs and PSCs were lysed and proteins subjected to immunoblotting using anti-FN antibody. GAPDH was used as a loading control. For PCCs, ponceau image also indicates loading. c Cancer cells seeded on 96-well plates with- or without FN-coating were incubated for 24 h prior to incubation with transport buffer containing [3H]-gemcitabine for 4 h. Data are mean ± SEM of triplicate determinations. FN, fibronectin; PCC, pancreatic cancer cell; PSC, pancreatic stellate cell