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Fig. 3 | BMC Cancer

Fig. 3

From: MiR-7-5p-mediated downregulation of PARP1 impacts DNA homologous recombination repair and resistance to doxorubicin in small cell lung cancer

Fig. 3

Impairment of HR repair by miR-7-5p in Dox-resistant SCLC cells. a Reporter construct used for the analysis of HR repair. After the introduction of DSBs by I-SceI digestion, HR repair was measured by GFP gene reconstitution in cells containing a single copy of a chromosomally integrated HR repair substrate (DR-GFP). b SCLC cells were cotransfected with I-SceI and pDRGFR plasmids, and then the percentage of GFP-positive cells was measured by FACS. c HR repair activity was impeded by miR-7-5p in SCLC cells. H446AR and H446 cells were transfected with miR-7-5p mimic or inhibitor, respectively, and the reporter construct was transiently transfected 24 h later, followed by the analysis of the GFP-positive cells by FACS. The mean ± SD of three independent experiments (right panel) and representative FACS profiles (left panel) are shown (b-c). *P < 0.05, **P < 0.01 and ***P < 0.001

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