Fig. 5

PI3K/AKT signaling pathway was involved in CDCA2-induced colon cancer cell proliferation. a Western blot was used to detected wheather PI3K/AKT pathway was involved in the regulation of proliferation under the influence of CDCA2. b CCND1 mRNA expression was detected in the indicated cell lines under the infuence of LY294002. c Western blotting was used to detect the relationship among CDCA2, PI3K/AKT pathway and CCND1. d, e and f Rescue experiments (colony formation and cck-8 rescue assays) were conducted to further confirm the above results