Fig. 2From: CDCA2 promotes the proliferation of colorectal cancer cells by activating the AKT/CCND1 pathway in vitro and in vivoExpression of CDCA2 in CRC cells and loss or gain of function assays. a The levels of CDCA2 mRNA expression were verified by RT-PCR in SW480 after treatment with siRNAs against CDCA2. b CDCA2 protein levels were measured by western blotting. c Colony formation assays were performed to determine the proliferation of SW480 and DLD-1 cells. Colonies were counted and captured. d Effects of CDCA2 alteration on the cell viability of CRC cells were detected by CCK-8 assay. e EdU staining assays were performed to determine the growth of SW480 and DLD-1 cells. Representative images and data are based on three independent experiments. * represents p < 0.05, ** represents p < 0.01, *** represents p < 0.001Back to article page