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Fig. 4 | BMC Cancer

Fig. 4

From: The expression of S100A8/S100A9 is inducible and regulated by the Hippo/YAP pathway in squamous cell carcinomas

Fig. 4

The Hippo pathway is responsible for S100A8/S100A9 induction. HCC94 cells were transfected with His-YAP-WT and His-YAP-S127A under suspension and dense culture condition (a). MST1 and LATS1 were knockdown by corresponding siRNAs in suspension and dense cultured HCC94 cells (b and c). Deletion of YAP in normal attached HCC94 and FaDu cells, the expression of S100A8/S100A9 was tested by western blot (d), and CTGF, CYR61 were detected by qPCR (e and f). Overexpression of LATS1 in normal attached HCC94 cells and FaDu cells (g), anti-flag tag antibody was used to judge the transfection efficiency. TEAD1 was deleted by two specific siRNAs in HCC94 (h) and FaDu (i) cells, the expression of S100A8/S100A9 was detected by western blot and the gray value of S100A8/S100A9 was analyzed by ImageJ Launcher. HCC94 cells (j) and FaDu cells (k) were transfected with Flag-YAP-WT and Flag-YAP-S94A plamids, anti-flag tag antibody was used to judge the transfection efficiency. YAP was not binding on S100A8/S100A9 promoter sites were detected by CHIP analysis using anti-YAP versus IgG control antibody, CYR61 was as positive control (l)

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