Fig. 2

Induction of BMCC1 following CDDP treatment in NB cells was positively correlated with phosphorylation of ATM. a CDDP stimulates the expression of BMCC1 while promoting the phosphorylation of ATM, Chk2, and E2F1. SK-N-AS cells were treated with 40 μM of CDDP. At regular time intervals after treatment, the cell lysates were prepared and immunoblotted. b and c CDDP-dependent accumulation of BMCC1 was abrogated by ATM inhibition. CDDP-treated SK-N-AS cells were cultured in the presence or absence of an ATM inhibitor. At regular time intervals after incubation with the inhibitor, the cell lysates and total RNA were prepared and subjected to immunoblotting (b) and semiquantitative RT-PCR (c), respectively