Skip to main content
Fig. 5 | BMC Cancer

Fig. 5

From: Deletion of sorting nexin 27 suppresses proliferation in highly aggressive breast cancer MDA-MB-231 cells in vitro and in vivo

Fig. 5

Confirmation the effect of SNX27 knockdown on E-cadherin activity by siRNA transfection. SNX27-KD and WT cells (50–60% confluence in 12-well plates) were transfected with E-cadherin siRNA. Lysates were blotted with anti-E-cadherin, anti-PCNA and anti-EMT-related markers. The SNX27-KD MDA-MB-231 cells transfected with E-cadherin siRNA were significantly decreased the E-cadherin expression compared with cells treated with control siRNA and transfection reagent control. The expression of PCNA, the cell proliferation marker, was increased in SNX27-KD cells transfected with E-cadherin siRNA. Data are analyzed with One-way ANOVA adjusting multiple comparisons with Turkey method, and expressed with mean ± SD from six independent experiments (n = 6). ns: no significantly difference, *p < 0.05, **p < 0.01, ***p < 0.001

Back to article page

BMC Cancer

ISSN: 1471-2407

Contact us