Fig. 3

The aggressiveness of SNX27-KD and WT MDA-MB-231 cells in soft agar. To investigate the influence of SNX27 on the aggressiveness of breast cancer MDA-MB-231 cells, the colony forming ability of SNX27-KD and WT MDA-MB-231 breast cancer cells, were observed by soft agar assays. The pictures taken under an inverted phase-contrast microscope with 4× (a) 20× (b) magnifier object. c SNX27 knockdown cells formed significantly fewer colonies than the parental MDA-MB-231 cells. Data are analyzed with Welch’s t-test, and expressed as mean ± SD from three independent experiments (n = 3). **p < 0.01 vs. WT control