Skip to main content
Fig. 4 | BMC Cancer

Fig. 4

From: LncRNA WWC2-AS1 functions AS a novel competing endogenous RNA in the regulation of FGF2 expression by sponging miR-16 in radiation-induced intestinal fibrosis

Fig. 4

lncRNA WWC2-AS1 is physically associated with miR-16 and functions as a ceRNA of FGF2. a MS2-RIP followed by RT-qPCR to detect endogenous miR-16 associated with WWC2-AS1. b Luciferase activity in CCD-18Co and SEMFs cells co-transfected with miR-16 and luciferase reporters containing nothing (pmirGLO), Wt-WWC2-AS1 or Mut-WWC2-AS1. c The mRNA levels of miR-16 were determined by RT-qPCR in CCD-18Co and SEMFs cells transfected with miR-16. d The mRNA levels of WWC2-AS1 in control (Con) and WWC2-AS1 overexpression (WWC2-AS1) cells were determined by RT-qPCR. e The protein levels of FGF2 in CCD-18Co and SEMFs transfected with control and WWC2-AS1 were determined by Western Blot. Statistical analysis was displayed in columns. f The relative mRNA levels of FGF2 in Wt or Mut WWC2-AS1 overexpressed cells with or without overexpression of miR-16. g The relative protein levels of FGF2 in Wt or Mut WWC2-AS1 overexpressed cells with or without miR-16. Statistical analysis was displayed in columns. h The relative luciferase activity of FGF2 3’UTR in Wt or Mut WWC2-AS1 overexpressed cells with or without of miR-16. *P < 0.05, **P < 0.01, compared to control group

Back to article page

BMC Cancer

ISSN: 1471-2407

Contact us