Fig. 5

Ras-ERK1/2 induced Williams–Beuren syndrome transcription factor (WSTF) to decrease H2A.X^Y142ph. a-d SNU-16 cells were transfected with pEGFP-K-Ras^G12V/T35S, pEGFP-N1, and WSTF-HA or EYA3-HA plasmids. 24 h later, RNA was collected and detected by PCR; 48 h later, cell lysates were analyzed using western blot. eThe recruitment of WSTF to the genes that exhibited changes in H2A.X ^Y142ph was analyzed using ChIP analysis. f Whole cell extracts from SNU-16 cells that were treated with MG132 were prepared, and the WSTF protein levels were analyzed using western blot with antibodies against HA and GFP. g-i Whole cell extracts from SNU-16 cells that were untreated (the samples were collected at 24, 48, 51, 54, and 60 h following transfection; upper panel) or treated with MG132 (the MG132was added at 48 h following transfection, and the samples were collected at 24, 48, 51, 54, and 60 h following transfection; lower panel) were analyzed using western blot. Data presented as mean + SD, * P < 0.05, ** P < 0.01, *** P < 0.001 (n = 3)