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Fig. 4 | BMC Cancer

Fig. 4

From: RETRACTED ARTICLE: K-ras-ERK1/2 down-regulates H2A.XY142ph through WSTF to promote the progress of gastric cancer

Fig. 4

Silence of eyes absent homolog (EYA) 3 up-regulated H2A.XY142ph. a The efficiency of siRNA-mediated EYA3 knockdown was determined. The depletion of EYA3 prevented the Ras-ERK1/2 activation-induced decrease in the H2A.XY142ph. SNU-16 cells were co-transfected as indicated. Whole cell lysates were assayed using western blot. SNU-16 cells were co-transfected with pEGFP-K-RasG12V/T35S or pEGFP-N1 plasmids and EYA3-specific or control siRNA as indicated (Ras, GFP, si-EYA3–1, si-EYA3–2, or si-con, respectively). b Cell viability, (c) cell migration, (d) cell cycle progression were detected by MTT assay, Transwell assays and flow cytometry, respectively. (e) Silence of EYA3 on downstream factors was detected by real time RCR. Data presented as mean + SD, * P < 0.05, ** P < 0.01, *** P < 0.001 (n = 3)

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