Fig. 2
From: ERO1α is a novel endogenous marker of hypoxia in human cancer cell lines
ERO1α and CA9 expression profiles in HCT116 and HeLa cells under normoxia and hypoxia. The expression of ERO1α and CA9 at the protein (a) and mRNA (b) levels were investigated over time in hypoxic cultures by western blotting (WB) and quantitative PCR (qPCR), respectively, using HCT116 cells (CA9-low) and HeLa cells (CA9-high). Cultured cells were incubated for 0, 6, 12, 24, 48, or 72 h under hypoxia. Cells incubated for 72 h under normoxia were used as a control. In WB analysis, β-actin and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were used as internal controls. HIF1α was detected to confirm hypoxia. In the qPCR analysis, the relative mRNA levels were calculated from the comparative threshold cycle (Ct) values relative to the actin B gene (ACTB). Data are presented as the mean ± the standard error of the mean (SEM, n = 4). *P < 0.05 calculated by ANOVA followed by a Bonferroni test. n.s.: not significant