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Fig. 6 | BMC Cancer

Fig. 6

From: Vaccination with synthetic long peptide formulated with CpG in an oil-in-water emulsion induces robust E7-specific CD8 T cell responses and TC-1 tumor eradication

Fig. 6

E7 SLP + CpG + o/w emulsion is optimal for robust CD8 T cell induction. Mice (n = 5) were immunized s.c. on day 0 and 21 with either 0.6, 3, or 15 μg E7 SLP or with 3 or 15 μg E7 ME peptide, in the presence of 20 μg CpG + o/w emulsion. Splenocytes were isolated on day 28. a E7 dextramer+ CD8 T cells were enumerated by FACS. b Cells were restimulated with E7 ME peptide for 5 h, then FACS analyzed for E7-specific multifunctional CD8 T cells intracellular expression of IFN-γ, TNF-α or IL-2. Two-sided hypothesis test for comparisons using one-way ANOVA model; *, p < 0.05; **, p < 0.01

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BMC Cancer

ISSN: 1471-2407

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