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Fig. 3 | BMC Cancer

Fig. 3

From: Vaccination with synthetic long peptide formulated with CpG in an oil-in-water emulsion induces robust E7-specific CD8 T cell responses and TC-1 tumor eradication

Fig. 3

E7 SLP + CpG + o/w emulsion induces a robust CD8 T cell expansion. Mice (n = 5) were immunized s.c. on day 0 and 21 with 5 μg E7 SLP adjuvanted with 20 μg CpG, 25 μg MPL, 50 μg poly(I:C), or 20 μg R848, and formulated in either w/o emulsion or o/w emulsion. Splenocytes were isolated on day 28. Cells were restimulated with E7 ME peptide for 5 h, then FACS analyzed for E7-specific multifunctional CD8 T cells. a Representative dot plots for intracellular expression of TNF-α and IFN-γ by CD8 T cells is presented b Individual polyfunctionality profiles with percentages of intracellular TNF-α, IL-2, and IFN-γ are presented for each condition

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BMC Cancer

ISSN: 1471-2407

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