Skip to main content
Fig. 2 | BMC Cancer

Fig. 2

From: A novel long non-coding RNA from the HOXA6-HOXA5 locus facilitates colon cancer cell growth

Fig. 2

Knockdown of HOXA5 short RNA inhibited cell growth. a and b (left panels) and c (left and right panels). After HCT116 cells were treated with 10 nM of the indicated siRNAs for 48 h, expression levels of HOXA5 mRNA, HOXA5 long RNA, and HOXA5 short RNA were measured by qPCR using GAPDH mRNA as an endogenous quantitative control. Data are expressed as the mean fold changes ± standard deviation (SD; n = 4) compared with those in the control siRNA-treated cells. *Statistically significantly difference versus control siRNA-treated (unpaired Student’s t-test, P < 0.05). a, b, and c (middle panels). HCT116 cells (1.0 × 105 cells) were seeded into 35-mm-diameter dishes and transfected with 10 nM of the indicated siRNAs or control siRNA. Subsequently, the growing cells were counted at the indicated times. Values are means ± SD (n = 4). *Statistically significantly difference versus control siRNA-treated (unpaired Student’s t-test, P < 0.01). a and b (right panels). After HCT116 cells were treated with 10 nM of the indicated siRNAs for 48 h, nuclear fractions were prepared from the cells. The levels of HOXA5 protein were measured by western blotting. The same amounts of protein used for western blotting were subjected to SDS-PAGE followed by CBB staining, for a loading control. The amount of HOXA5 protein relative to that of CBB stained bands was quantitatively analyzed by densitometry

Back to article page