Fig. 4
From: Sigma-2 receptor/TMEM97 agonist PB221 as an alternative drug for brain tumor
The effect of anti-oxidant on PB221 effects on ALTS1C1 (a) A summary graph reveals the level of mitochondria superoxide in brain tumor cell lines, U87-MG and ALTS1C1 following PB221 stimulation was measured by the fluorescent intensity of MitoSoxTM Red using flow cytometry. (b) Represented pictures of migration and invasion assays illustrate the effect of anti-oxidant α-tocopherol and NAC on PB221 (1 μM)-inhibited migration and invasion ability of ALTS1C1 cells. Scale bar = 100 μm. (c) A summary graph shows the preventing effect of 10 μM of α-tocopherol, but not NAC, on PB221 (1 μM)-inhibited migration and invasion ability of ALTS1C1 cells. (d) A summary graph reveals the preventing effect of 10 μM of α-tocopherol, but not NAC, on PB221 (20 μM)-induced apoptotic cell death of ALTS1C1 cells assayed by flow cytometry. *: P < 0.05. **: P < 0.01. ***: P < 0.001