Fig. 1

The responses of brain and pancreatic cell lines to the cytotoxicity of PB221 and binding affinity with selective sigma-2 receptor agonist ligand, NO1. (a) The survival curve of various cell lines in response to a range of PB221 and PB28 concentration for 3 days was measured by MTT assay. The IC₅₀ for murine astrocytoma ALTS1C1 cells and murine pancreatic UN-KC6141 cells was 10.61 and 13.13 μM, respectively. ALTS1C1* open circle curve is the responsive curve of ALTS1C1 to PB28 and the IC₅₀ is 11.78 μM. (b) The binding affinity of ALTS1C1 and UN-KC6141 cells with selective sigma-2 receptor ligand, NO1, was examined by fluorescent plate reader and the relative fluorescent unit (RFU) was measured with the excitation of 390 nm and emission at 525 nm