Fig. 6
Local correlations between cellular glycogen using RS and CAIX Immunofluorescence. Raman intensity maps of glycogen (PC1), indicating the spatial distribution (20 × 20 μm2 pixel size) of glycogen in a 0 Gy (a) and 15 Gy (c) tissue section (harvested at 3 days). Coincident CAIX immunofluorescence intensity spatial maps (20 × 20 μm2 pixel size) for the same region are also shown for 0 Gy (b) and 15 Gy (d). Bright regions indicate high CAIX stain intensity or high glycogen (PC1 score) at that pixel (e-f). The mean CAIX intensity for 100 × 100 μm2 regions within each intensity map is plotted against the mean PC1 score in the same region (error bars are the standard deviation), indicating a general correlation of high CAIX intensity linked to high PC1 scores