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Fig. 5 | BMC Cancer

Fig. 5

From: Raman spectroscopy detects metabolic signatures of radiation response and hypoxic fluctuations in non-small cell lung cancer

Fig. 5

Trends in CAIX expression between irradiated and unirradiated tumours harvested at 3 days post-treatment. Immunofluorescence images showing nuclear localization through DAPI (blue) and surrogate marker for hypoxia through CAIX (yellow) for 0 Gy (a) and 15 Gy (b) tumours. The mean percent area positive for CAIX over several images per dose group (n = 136 for 0 Gy, n = 122 for 15 Gy) is shown in (c), indicating a statistically significant increase in CAIX staining for irradiated tumours (**p ≤ 0.01). Error bars indicate standard error on the mean. (d) Median PC1 score indicating glycogen content is plotted against the mean percent CAIX positive for each 0 Gy and 15 Gy tumour studied at 3 days post-treatment. A linear correlation between glycogen content (PC1 score) and percent CAIX positive for a given tumour is shown (R2 = 0.90, p = 0.01)

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