Fig. 2
From: Lineage relationship between prostate adenocarcinoma and small cell carcinoma
Transfected 293F cells. a The photomicrographs show confluent untransfected 293F cells, confluent 293F transfected with an IgG1 plasmid, 293F cells transfected with scTF plasmids. Magnification 50X. b Gene expression of scTF-transfected 293F* cells: 630 bp LIN28A, 930 bp NANOG, 1100 bp POU5F1, 960 bp SOX2 (size difference to the corresponding ones in Fig. 1 is due to different primers). c Gene expression of IgG-transfected 293F cells (720 bp L chain; 1420 bp H chain). The level of B2M in scTF-transfected cells is lower than that in IgG1-transfected cells (compared to those of neo). d Shown are 293F* colonies on different culture media formulations: MEF + KSR, Matrigel + KSR, CM, KSR. Magnification 50X