Fig. 2

4EBP1 is highly expressed and phosphorylated in 8p11-p12 breast cancer cells. (a) Western blot of 4EBP1 and phospho-4EBP1 on residues Thr 37/37, Thr 70, and Ser 65 in SUM-44 (ER+), Cama-1 (ER+), and SUM-52 (ER-) cells with amplification of the 8p11-p12 genomic locus (8p11-12 CNA) as well as T47D (ER+), HCC1500 (ER+), MCF7 (ER+), and SUM-229 (ER-) cells without amplification of the 8p11-p12 genomic locus. Immortalized but non-tumorigenic breast epithelial cells are represented by MCF10A and H16N2 cells. (b) Cell proliferation was assessed in SUM-44, Cama-1, and SUM-52 cells in the presence or absence of 1 μM everolimus treatment for 72 h. Error bars represent standard deviation among replicates and p values are for the difference in cell proliferation in control versus treated cells. The p-value for the difference between the effect in SUM-52 cells and the other cell lines is <0.0001