Fig. 3

Knockdown of MVP promotes LLC cell proliferation. a Representative images of colony formation of the transfected LLC cells. Cells were seeded and cultured for 14 days. After fixed and stained, the cell colonies were counted. b Quantification of the colony formation. (n = 3, ** P < 0.01). c. Representative immunofluorescence staining of Edu and DAPI in the transfected LLC cells. Cells were treated with serum-free medium for 24 h, regular medium for 24 h, and regular medium plus the Edu for 2 h. Cells were then fixed with 4% PFA and stained with DAPI. d Quantification of the Edu staining. (n = 3, ** P < 0.01). e Cell cycle profiles of the lenti-shRNA stablely transfected LLC cells. Cells were harvested, washed with PBS and fixed in 70% ethanol at 4 °C for at least 12 h. After washed in PBS, stained with PI for 30 min and resuspended in PBS, cells were measured by a flow cytometry. f, g Quantification of the cell cycle profiles. (n = 3, ** P < 0.01). h Representative western blot of the indicated proteins in the transfected LLC cells. i, j Quantification of the western blot. (n = 3, * P < 0.05)