Fig. 1
p16 staining in epithelioid malignant pleural mesothelioma (MPM-E). Both the whole section (a, c, and e; using CINtec® p16 Histology clone E6H4™ with DAP as chromogen) and tissue array (TMA) cylinder samples (b, d, and f; using mouse monoclonal antibody for p16 [JC8] with AEC as chromogen) are shown. All microscopic illustrations have magnification × 40. Panels a) and b) show p16 staining in the case E-161 who had pulmonary asbestos fiber count of 0.4 × 106 fibers / gram dry weight (f/g). Tumor cells showed positivity of +++ (a) to ++ (b) while stromal cells (arrows) were negative for p16. Panels c) and d) show p16 staining in the case E-143 who had pulmonary asbestos fiber count of 8.9 × 106 f/g. Tumor cells showed strong positivity and stromal cells had positivity of ++ (c) to + (d). Panels e) and f) show p16 staining in the case E-250 who had pulmonary asbestos fiber count of 13.0 × 106 f/g. Tumor cells were negative for p16 whereas stromal cells showed positivity of +++ (e) to ++ (f)