Fig. 5
From: Oncogenic Ras is downregulated by ARHI and induces autophagy by Ras/AKT/mTOR pathway in glioblastoma
CQ can promote apoptosis in ARHI-glioma cells. a-b Cell viability of LN229 and T98G cells transfected with ARHI and negative control plasmids cultured with 2 mM 3-MA or 3 μM CQ after 48 h. c-d Western blot showing SQSTM1, LC3, Bcl2, Ras and ARHI levels in LN229 cells after treatment with 2 mM 3-MA or 3 μM CQ for 48 h. The experiments were repeated 3 times independently. e-h After treatment with 3-MA or CQ, LN229 cells were analyzed by flow cytometry and examined by western blot for apoptosis-related caspase-3 protein. All experiments were repeated 3 times independently